XTT sodium salt is widely used in cell biology and biochemistry for assessing cell viability and proliferation. It serves as a key component in the XTT assay, where it is reduced by metabolically active cells to form a water-soluble formazan dye. This colorimetric change is measured spectrophotometrically, providing a quantitative measure of cell health. The assay is particularly useful for high-throughput screening in drug discovery, cytotoxicity studies, and evaluating the effects of various compounds on cell growth. Its water-soluble nature eliminates the need for additional solubilization steps, making it a convenient choice for researchers. Additionally, XTT sodium salt is applied in microbiology to study microbial metabolic activity and in cancer research to test the efficacy of anticancer agents.