L-Alanine 4-nitroanilide hydrochloride
99%
- Product Code: 98033
Alias:
4-Nitroaniline hydrochloride-L-alanine
CAS:
31796-55-1
| Molecular Weight: | 245.66 g./mol | Molecular Formula: | C₉H₁₁N₃O₃HCl |
|---|---|---|---|
| EC Number: | 250-811-0 | MDL Number: | MFCD00039088 |
| Melting Point: | Boiling Point: | ||
| Density: | Storage Condition: | -20°C |
Product Description:
L-Alanine 4-nitroanilide hydrochloride is primarily used in biochemical research as a substrate for studying enzyme activity, particularly proteases and peptidases. It serves as a chromogenic substrate, enabling the detection and quantification of enzyme activity through colorimetric assays. When the enzyme cleaves the compound, it releases 4-nitroaniline, which produces a yellow color that can be measured spectrophotometrically. This makes it a valuable tool in enzymology for investigating enzyme kinetics, inhibition, and specificity. Additionally, it is utilized in diagnostic applications to assess enzyme function in clinical and laboratory settings.
Product Specification:
| Test | Specification |
|---|---|
| Purity (HPLC) | 99-100% |
| Water (Karl Fischer) | 0-1% |
| Appearance | Yellow powder |
| Infrared Spectrum | Conforms to Structure |
| Solubility in Water | Yellow clear solution (50MG/ML) |
Sizes / Availability / Pricing:
| Size | Availability | Price | Quantity |
|---|---|---|---|
| 250mg | 10-20 days | ฿2,770.00 |
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| 1g | 10-20 days | ฿7,290.00 |
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L-Alanine 4-nitroanilide hydrochloride
L-Alanine 4-nitroanilide hydrochloride is primarily used in biochemical research as a substrate for studying enzyme activity, particularly proteases and peptidases. It serves as a chromogenic substrate, enabling the detection and quantification of enzyme activity through colorimetric assays. When the enzyme cleaves the compound, it releases 4-nitroaniline, which produces a yellow color that can be measured spectrophotometrically. This makes it a valuable tool in enzymology for investigating enzyme kinetics, inhibition, and specificity. Additionally, it is utilized in diagnostic applications to assess enzyme function in clinical and laboratory settings.
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| Stable in pH range | - |
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| INCI | - |
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