6-Trifluoromethoxychromone-2-carboxylic acid is primarily utilized in the field of medicinal chemistry and drug development. It serves as a key intermediate in the synthesis of various bioactive compounds, particularly those targeting inflammatory and oxidative stress-related diseases. Its structural framework is often incorporated into molecules designed to inhibit specific enzymes or pathways involved in these conditions. Researchers also explore its potential in creating novel fluorinated chromone derivatives, which are studied for their enhanced stability and biological activity. Additionally, it finds application in the development of fluorescent probes for biochemical assays, leveraging its chromone core for optical properties.