This chemical is primarily used in bioconjugation applications, particularly in the field of bioorthogonal chemistry. Its tetrazine group enables rapid and selective reactions with strained alkenes or alkynes, making it valuable for labeling biomolecules such as proteins, antibodies, or nucleic acids. The polyether chain in its structure enhances water solubility, facilitating its use in biological systems. It is often employed in live-cell imaging, drug delivery systems, and the development of diagnostic tools, where precise and efficient labeling is crucial. Additionally, its carboxylic acid group allows for further functionalization or conjugation to other molecules, expanding its utility in chemical biology and materials science.