This chemical is widely used in bioconjugation and click chemistry applications due to its unique functional groups. The azide group enables it to participate in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, while the propargyl group serves as a complementary partner in these reactions. Its PEG12 spacer provides excellent solubility, reduced immunogenicity, and enhanced biocompatibility, making it suitable for modifying biomolecules like proteins, peptides, and antibodies. It is particularly useful in drug delivery systems, where it helps in creating stable and targeted conjugates. Additionally, it is employed in the development of diagnostic probes, biolabeling, and surface functionalization of nanoparticles for biomedical research.