This compound is primarily utilized in the field of bioorthogonal chemistry, where it serves as a key reagent for click chemistry reactions. Its ability to undergo inverse electron-demand Diels-Alder reactions with strained alkenes or alkynes makes it highly effective for labeling and modifying biomolecules in live cells without interfering with native biological processes. It is particularly valuable in the development of targeted drug delivery systems, where it facilitates the attachment of therapeutic agents to specific biomolecules. Additionally, it is employed in the synthesis of fluorescent probes for imaging and tracking cellular processes in real-time. Its versatility also extends to materials science, where it is used to create functionalized polymers and advanced materials with tailored properties.