FAM azide, 6-isomer is widely used in bioconjugation and labeling applications due to its reactive azide group and fluorescent properties. It is particularly valuable in click chemistry, where it participates in copper-catalyzed or strain-promoted azide-alkyne cycloaddition reactions to label biomolecules such as proteins, nucleic acids, and lipids. Its fluorescence makes it suitable for imaging and tracking in live cells or fixed tissues, aiding in studies of cellular processes and molecular interactions. Additionally, it is employed in the development of diagnostic assays and probes for detecting specific biomolecules. Its stability and high reactivity make it a preferred choice for researchers in biochemistry and molecular biology.