Cyanine5 DBCO is widely used in bioimaging and bioconjugation applications due to its near-infrared fluorescence properties and the ability to undergo click chemistry reactions. It is particularly valuable in labeling biomolecules such as proteins, antibodies, and nucleic acids for live-cell imaging, as its emission wavelength minimizes background autofluorescence and allows for deep tissue penetration. The DBCO (dibenzocyclooctyne) group enables strain-promoted azide-alkyne cycloaddition (SPAAC) reactions, facilitating efficient and selective conjugation with azide-containing molecules without the need for catalysts. This makes it a powerful tool in drug delivery systems, diagnostic assays, and the development of targeted therapeutics. Additionally, its stability and high fluorescence quantum yield make it suitable for tracking cellular processes and studying molecular interactions in complex biological systems.