DBCO-Palmitic is widely used in bioconjugation and chemical biology research due to its unique properties. The DBCO (dibenzocyclooctyne) group enables rapid and selective reactions with azide-containing molecules via strain-promoted alkyne-azide cycloaddition (SPAAC), a click chemistry reaction. This makes it valuable for labeling biomolecules, such as proteins, lipids, and nucleic acids, without the need for catalysts or harsh conditions. The palmitic acid moiety allows for hydrophobic interactions, making it particularly useful for studying lipid metabolism, membrane dynamics, and lipid-protein interactions. It is commonly employed in the development of lipid-based drug delivery systems, fluorescent labeling of cell membranes, and tracking lipid modifications in cellular processes. Its applications also extend to the creation of bioorthogonal probes for imaging and diagnostics in live cells and organisms.