BTTAA is widely utilized as a ligand in click chemistry, particularly in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. Its primary application lies in facilitating efficient and biocompatible conjugation of molecules, making it valuable in bioconjugation, drug discovery, and material science. BTTAA enhances the stability and solubility of copper catalysts, enabling reactions to proceed under mild conditions, which is crucial for sensitive biological systems. It is also employed in the development of bioconjugated probes, labeling biomolecules for imaging and diagnostic purposes. Additionally, BTTAA is used in the synthesis of polymers and nanomaterials, where precise molecular assembly is required. Its role in improving reaction efficiency and reducing cytotoxicity makes it a preferred choice in biomedical research and therapeutic applications.