MBTH indicator is widely used in biochemical and analytical chemistry for the detection and quantification of various compounds. It is particularly effective in colorimetric assays, where it reacts with specific substances to produce a measurable color change. This makes it a valuable tool in the analysis of phenols, amines, and other organic compounds in environmental and industrial samples. In microbiology, MBTH is employed to detect the presence of certain enzymes, such as oxidases, by forming colored complexes that can be easily observed and quantified. This application is crucial in studying microbial activity and enzyme kinetics. Additionally, MBTH is used in the pharmaceutical industry for the determination of drug compounds and their metabolites. Its ability to form stable, colored complexes with specific analytes allows for precise and sensitive measurements, essential for quality control and research purposes. In environmental monitoring, MBTH helps in detecting pollutants like phenolic compounds in water and soil samples. Its sensitivity and specificity make it a reliable choice for assessing environmental contamination and ensuring compliance with regulatory standards.