Tetrahydrocortisone is primarily used in medical and biochemical research to study cortisol metabolism and its effects on the body. It serves as a metabolite of cortisol, helping researchers understand the breakdown pathways and hormonal regulation in various physiological processes. This compound is also utilized in diagnostic testing to assess adrenal gland function and to detect abnormalities in steroid metabolism. Additionally, it plays a role in developing treatments for conditions related to cortisol imbalance, such as Cushing's syndrome or Addison's disease. Its applications extend to pharmacology, where it aids in the development of drugs targeting steroid-related disorders.