4-Methoxycoumarin is widely used in the fragrance and flavor industry due to its pleasant, sweet, and hay-like aroma. It serves as a key ingredient in perfumes, cosmetics, and scented products to enhance their olfactory profiles. In addition, it is utilized in the food industry as a flavoring agent, imparting a unique taste to various products.

In the field of organic chemistry, it acts as a versatile intermediate for synthesizing more complex coumarin derivatives, which have applications in pharmaceuticals and agrochemicals. Its photochemical properties make it valuable in UV-absorbing formulations, such as sunscreens and protective coatings.

Moreover, 4-Methoxycoumarin is studied for its potential biological activities, including antioxidant and anti-inflammatory effects, which could be harnessed in medicinal research and drug development. Its ability to interact with enzymes and proteins also makes it a subject of interest in biochemical studies.