This chemical is widely used as a fluorescent probe in biochemical and cellular studies due to its strong fluorescence properties. It is particularly valuable in detecting and quantifying reactive oxygen species (ROS) in biological systems, making it a crucial tool in oxidative stress research. Additionally, it serves as a sensitive marker for monitoring enzyme activities, such as esterases and lipases, in various assays. Its application extends to the development of biosensors, where it helps in the detection of specific biomolecules with high precision. In pharmaceutical research, it is utilized to study drug interactions and metabolic pathways, providing insights into drug efficacy and safety.