Sulfo-SANPAH is widely used in biochemical and biomedical research for crosslinking proteins to surfaces or other molecules. It is particularly valuable in immobilizing proteins onto solid supports, such as glass or plastic, for applications like ELISA assays, biosensor development, and cell adhesion studies. Its water-soluble nature and photoreactive properties allow for efficient and controlled crosslinking under mild conditions, making it ideal for preserving protein function and structure. Additionally, Sulfo-SANPAH is employed in studying protein-protein interactions and creating stable protein arrays for high-throughput screening. Its versatility and effectiveness make it a critical tool in advancing research in proteomics, cell biology, and material science.