N,N'-Methylenebis(acrylamide) is widely used as a crosslinking agent in the production of polyacrylamide gels, which are essential in electrophoresis for separating proteins and nucleic acids based on size. It is also employed in the synthesis of hydrogels for biomedical applications, including drug delivery systems and tissue engineering, due to its ability to form stable, water-absorbent networks. In the cosmetics industry, it is utilized in formulations for hair styling products, providing long-lasting hold and structure. Additionally, it finds use in the production of water treatment chemicals, where it helps in flocculation and purification processes by enhancing the efficiency of polymer chains in trapping impurities.