EEDQ is primarily used in biochemical research as a reagent for modifying proteins and peptides. It acts as a coupling agent, facilitating the formation of amide bonds between amino acids, which is essential for synthesizing peptides in laboratory settings. Additionally, EEDQ is employed in studies investigating receptor-ligand interactions, as it can irreversibly block certain receptors, helping researchers understand their function and role in biological processes. Its ability to crosslink proteins also makes it useful in probing protein-protein interactions and structural studies.