Benzo-15-crown-5 is widely used in chemical research and industrial applications due to its ability to selectively bind with certain metal ions, particularly potassium. This property makes it valuable in the development of ion-selective electrodes, which are crucial for measuring potassium ion concentrations in various samples, including biological fluids and environmental water.

In organic synthesis, it serves as a phase-transfer catalyst, facilitating the movement of ions between immiscible phases, thereby enhancing reaction rates and yields. Additionally, it is employed in the extraction and separation of metal ions from complex mixtures, aiding in purification processes.

Its role in supramolecular chemistry is also notable, as it is used to construct host-guest complexes, which are essential for studying molecular recognition and designing functional materials.