Used primarily in microbiology to cultivate anaerobic microorganisms, which cannot grow in the presence of oxygen. It provides a suitable environment for the isolation and identification of bacteria such as Clostridium and Bacteroides species. The medium supports growth by incorporating reducing agents that lower oxygen tension, ensuring optimal anaerobic conditions. Commonly used in clinical labs for diagnosing infections like abscesses, wound infections, and bacteremia caused by obligate anaerobes. Also utilized in environmental and food microbiology to study anaerobic bacterial populations.