Used primarily in microbiology for the cultivation of fastidious organisms, especially mycoplasmas and other pleuropneumonia-like organisms (PPLO). It supports the growth of these organisms due to its rich nutrient composition, including amino acids, vitamins, and nucleic acid derivatives. Commonly used in diagnostic laboratories for isolation and identification of mycoplasma from clinical samples such as respiratory secretions, urogenital swabs, and cell cultures. Also employed in research settings to study mycoplasma physiology and pathogenicity. The broth is often supplemented with serum and antibiotics to enhance selectivity and growth yield.