This compound is widely used as a fluorescent dye in biological research, particularly for staining cellular membranes. It is highly effective for labeling live cells, allowing researchers to visualize and study membrane potential and dynamics. Due to its lipophilic nature, it easily integrates into lipid bilayers, making it a valuable tool for tracking membrane changes in real-time. Additionally, it is employed in flow cytometry and fluorescence microscopy to assess cell viability and monitor mitochondrial activity. Its ability to emit strong fluorescence under specific wavelengths makes it a preferred choice for high-resolution imaging and diagnostic applications in cell biology.