Used extensively in fluorescence microscopy and cell biology for membrane labeling due to its strong lipophilic properties. It integrates into cell membranes and allows long-term cell tracking, making it ideal for monitoring cell migration, fusion, and proliferation. Commonly applied in neuroscience for neuronal tracing and in developmental biology for visualizing tissue formation. Its bright fluorescence and photostability support high-resolution imaging, including confocal and two-photon microscopy. Also utilized in flow cytometry for cell population analysis.