Used in bioorthogonal labeling applications, enabling specific and efficient conjugation to azide-containing biomolecules without interfering with biological processes. Its near-infrared fluorescence makes it ideal for live-cell imaging, allowing real-time tracking of cellular components with minimal background autofluorescence. Commonly employed in fluorescence microscopy, flow cytometry, and in vivo imaging due to its high photostability and brightness. The DBCO group facilitates copper-free click chemistry, making it suitable for sensitive biological systems where copper toxicity is a concern. Also utilized in multiplex assays when combined with other fluorophores, supporting simultaneous detection of multiple targets.