Used as a fluorescent dye in biological imaging and staining applications. Its strong fluorescence and water solubility make it suitable for labeling biomolecules and cellular structures, particularly in fluorescence microscopy and flow cytometry. The compound’s sulfonate groups enhance solubility and reduce nonspecific binding, improving signal clarity in complex biological samples. It is also employed in diagnostic assays and bioconjugation techniques where high sensitivity and photostability are required.