Used extensively as a fluorescent dye in biological imaging and cell biology research. It is particularly effective in staining cell membranes, allowing for detailed visualization under fluorescence microscopy. The dye is also employed in flow cytometry to study cell populations and membrane dynamics. Its high photostability and bright fluorescence make it suitable for long-term imaging studies. Additionally, it is utilized in tracking lipid rafts and membrane fusion processes in live cells. The compound's ability to integrate into lipid bilayers without disrupting their structure is a key advantage in membrane-related studies.