This chemical is primarily used as a fluorescent dye in various biological and chemical applications. It is commonly employed in fluorescence microscopy and flow cytometry to label and track cells, proteins, or other biomolecules due to its strong and stable fluorescence properties. Additionally, it is utilized in biochemical assays to study molecular interactions, such as protein-ligand binding or nucleic acid hybridization. Its water solubility, derived from the sulfonated groups, makes it suitable for use in aqueous environments, which is essential for biological research. The dye is also applied in diagnostic tools and imaging techniques, aiding in the visualization of cellular processes and structures. Its high sensitivity and photostability make it a valuable tool in both research and clinical diagnostics.