ICG Carboxylic Acid is widely used in biomedical imaging due to its near-infrared fluorescence properties. It is particularly valuable in fluorescence-guided surgeries, where it helps surgeons visualize and differentiate between healthy and diseased tissues in real-time. This compound is also employed in the development of contrast agents for optical imaging, enhancing the detection of tumors and other abnormalities. Additionally, it is utilized in research for labeling biomolecules, enabling the study of cellular processes and interactions. Its stability and biocompatibility make it a preferred choice in various diagnostic and therapeutic applications.