Cy7.5 carboxylic acid is widely used in biomedical research for labeling biomolecules due to its near-infrared fluorescence properties. It is particularly valuable in imaging applications, as its emission wavelength minimizes background interference from biological tissues, enabling deep tissue penetration and high-resolution imaging. This compound is often conjugated to antibodies, peptides, or proteins for targeted imaging in vivo, aiding in the visualization of tumors, inflammation, or other disease markers. Additionally, it is employed in the development of fluorescent probes for studying cellular processes and molecular interactions. Its stability and brightness make it a preferred choice for long-term tracking and real-time monitoring in preclinical studies.