Used primarily as a fluorescent dye in biological research, this chemical is valuable for staining cellular components, particularly mitochondria, due to its ability to penetrate cell membranes and emit strong fluorescence. It is widely applied in fluorescence microscopy and flow cytometry to study cell viability, membrane potential, and mitochondrial function. Additionally, it serves as a probe in assays to monitor apoptosis and other cellular processes, providing insights into cellular health and dynamics. Its stability and bright fluorescence make it a reliable tool in live-cell imaging and diagnostic applications.