Cyanine3 is widely used in biological research as a fluorescent dye for labeling proteins, nucleic acids, and other biomolecules. Its application is prominent in fluorescence microscopy, where it helps visualize cellular structures and processes with high specificity and brightness. It is also utilized in flow cytometry to detect and sort cells based on fluorescent markers. In molecular biology, Cyanine3 is employed in techniques like fluorescence in situ hybridization (FISH) to locate and identify specific DNA or RNA sequences within cells. Additionally, it plays a key role in immunoassays, where it labels antibodies to detect target antigens with high sensitivity. Its fluorescence properties make it suitable for various imaging applications, enabling researchers to track biological activities.