1-Aminobenzotriazole is primarily used as a selective inhibitor of cytochrome P450 enzymes in biochemical and pharmacological research. It is particularly valuable in studies aimed at understanding the role of these enzymes in drug metabolism, toxicity, and carcinogenesis. By inhibiting cytochrome P450, it helps researchers identify the specific metabolic pathways of drugs and xenobiotics, providing insights into potential drug-drug interactions and the mechanisms of chemical toxicity. Additionally, it is employed in in vitro and in vivo experiments to investigate the bioactivation of pro-carcinogens and the detoxification processes within the body. Its application extends to the development of safer pharmaceuticals and the assessment of environmental chemicals' impact on human health.