Butyl oxamate is primarily utilized in biochemical research as an inhibitor of lactate dehydrogenase (LDH), an enzyme involved in anaerobic glycolysis. By inhibiting LDH, it helps study the metabolic pathways in cells, particularly in cancer research, where altered metabolism is a key characteristic. It is also employed in enzymatic assays to understand the role of LDH in various physiological and pathological processes. Additionally, butyl oxamate has been explored for its potential therapeutic applications in conditions where LDH activity is dysregulated, such as certain types of cancer and metabolic disorders. Its ability to modulate LDH activity makes it a valuable tool in both basic science and drug development studies.