Used extensively in molecular biology for protein denaturation, aiding in the study of protein structure and function. It is a key component in RNA extraction protocols, helping to isolate RNA from cells by breaking down cell membranes and inactivating nucleases. In biochemistry, it serves as a strong chaotropic agent, disrupting hydrogen bonds and hydrophobic interactions, which is crucial for solubilizing proteins and nucleic acids. Additionally, it is employed in the purification of recombinant proteins, particularly in inclusion body isolation and refolding processes. Its ability to stabilize enzymes and other proteins makes it valuable in various biotechnological applications.