PAMAM dendrimers with an ethylenediamine core, particularly generation 6.0, are widely utilized in drug delivery systems due to their well-defined structure and ability to encapsulate or bind therapeutic agents. Their high surface functionality allows for the attachment of targeting molecules, enhancing the precision of drug delivery to specific cells or tissues. In gene therapy, these dendrimers are employed to condense and protect nucleic acids, facilitating their transport into cells for gene expression or silencing. Additionally, they are used in diagnostic imaging as carriers for contrast agents, improving the clarity and specificity of imaging techniques. Their biocompatibility and versatility also make them valuable in the development of biosensors and as scaffolds in tissue engineering, promoting cell growth and regeneration.