PAMAM-OH dendrimers are widely used in drug delivery systems due to their ability to encapsulate and transport therapeutic agents. Their highly branched structure allows for efficient loading of drugs, while their surface hydroxyl groups enable functionalization for targeted delivery. These dendrimers are also employed in gene therapy as non-viral vectors for DNA and RNA delivery, enhancing transfection efficiency. In biomedical imaging, they serve as contrast agents by attaching imaging moieties to their surface. Additionally, PAMAM-OH dendrimers are utilized in diagnostic applications for detecting biomarkers and in tissue engineering for scaffold modifications to promote cell growth and differentiation.