2-Deoxy-D-glucose 6-phosphate disodium is primarily used in biochemical research to study metabolic pathways, particularly glycolysis. It serves as a competitive inhibitor of glucose metabolism, allowing scientists to investigate the effects of glucose deprivation on cellular processes. This compound is also utilized in cancer research to explore the altered metabolic behavior of cancer cells, as they often rely heavily on glycolysis for energy production. Additionally, it is employed in studies related to diabetes and insulin resistance, helping to understand glucose uptake and utilization in various tissues. Its application extends to the development of therapeutic strategies targeting metabolic disorders and cancer.