Dinitrophenyl DL glutamic acid is primarily used in biochemical research as a reagent for studying enzyme activity and protein interactions. It serves as a substrate or inhibitor in enzymatic assays, particularly those involving glutamic acid-related enzymes. This compound is also utilized in the development of analytical methods for detecting and quantifying amino acids in complex biological samples. Additionally, it plays a role in immunological studies, where it can be conjugated to proteins or peptides to investigate antibody-antigen interactions. Its unique properties make it valuable in exploring metabolic pathways and understanding the mechanisms of amino acid metabolism.