ML216 is primarily utilized in biochemical research as a potent and selective inhibitor of the enzyme USP1 (Ubiquitin Specific Peptidase 1). Its application is significant in the study of DNA repair mechanisms, particularly in the context of cancer research. By inhibiting USP1, ML216 disrupts the deubiquitination process, which is crucial for the regulation of proteins involved in DNA damage response. This makes it a valuable tool for understanding how cancer cells manage DNA repair and for exploring potential therapeutic strategies that target DNA repair pathways. Researchers also use ML216 to investigate the role of USP1 in other cellular processes, such as cell cycle regulation and the maintenance of genomic stability. Its specificity and efficacy in inhibiting USP1 make it a critical compound in the development of novel cancer treatments and in advancing the broader field of molecular biology.