ML224 is a potent and selective inhibitor of the enzyme USP7, which plays a critical role in regulating various cellular processes, including tumor suppression and immune response. Its primary application lies in cancer research, where it is used to study the role of USP7 in tumor growth and progression. By inhibiting USP7, ML224 can help researchers understand how disrupting this enzyme affects the stability of key proteins involved in cancer development, such as p53. This makes it a valuable tool for exploring potential therapeutic strategies targeting USP7 in oncology. Additionally, ML224 is utilized in biochemical assays to investigate the broader biological functions of USP7 in cellular pathways, providing insights into its role in diseases beyond cancer, such as neurodegenerative disorders. Its specificity and potency make it a key compound in drug discovery and molecular biology studies.