ML364 is primarily utilized in research settings to study the ubiquitin-proteasome system, particularly its role in protein degradation and cellular homeostasis. It functions as an inhibitor of the deubiquitinase USP2, making it a valuable tool for investigating the mechanisms of protein turnover and the potential therapeutic targeting of diseases linked to dysregulated protein degradation, such as certain cancers. Researchers employ ML364 to explore how inhibiting USP2 affects tumor growth and survival, providing insights into novel cancer treatment strategies. Additionally, it is used in biochemical assays to better understand the broader implications of deubiquitination in cellular processes. Its application is largely confined to preclinical studies, where it aids in identifying pathways that could be modulated for therapeutic benefit.