Ubiquitin Isopeptidase Inhibitor I, G5, is primarily utilized in biochemical research to study the ubiquitin-proteasome pathway, a critical system for protein degradation in cells. By inhibiting isopeptidases, it helps researchers understand the role of ubiquitin in regulating protein turnover, cell cycle progression, and signal transduction. This compound is particularly valuable in investigating diseases related to protein misfolding or dysregulation, such as cancer and neurodegenerative disorders. It also aids in exploring potential therapeutic targets by modulating ubiquitin-dependent processes.