UKI-1 is primarily utilized in biochemical research as a selective inhibitor of the enzyme UCH-L1 (Ubiquitin C-terminal Hydrolase L1). This enzyme plays a significant role in the ubiquitin-proteasome system, which is crucial for protein degradation and regulation within cells. Researchers employ UKI-1 to study the effects of UCH-L1 inhibition on cellular processes, particularly in the context of neurodegenerative diseases like Parkinson's disease, where UCH-L1 activity is often implicated. By inhibiting UCH-L1, UKI-1 helps scientists understand the enzyme's role in protein aggregation and neuronal survival, providing insights into potential therapeutic strategies for treating such conditions. Additionally, UKI-1 is used in experimental models to explore the broader implications of ubiquitin pathway modulation in cancer and other diseases linked to protein homeostasis.