USP7-IN-1 is primarily utilized in research settings to study its inhibitory effects on the ubiquitin-specific protease 7 (USP7) enzyme. This enzyme plays a critical role in regulating protein degradation and stability within cells, particularly affecting pathways involved in cancer, neurodegenerative diseases, and immune responses. By inhibiting USP7, researchers can investigate its impact on tumor suppressor proteins like p53, which are often destabilized in cancer cells. This makes USP7-IN-1 a valuable tool for exploring potential therapeutic strategies in oncology. Additionally, it is used to understand the role of USP7 in cellular processes such as DNA repair, apoptosis, and epigenetic regulation, providing insights into disease mechanisms and drug development.