USP25 and 28 inhibitor AZ-1 is primarily used in research to study the role of deubiquitinating enzymes (DUBs) in cellular processes. It specifically targets USP25 and USP28, which are involved in regulating protein stability and signaling pathways. By inhibiting these enzymes, AZ-1 helps researchers understand their impact on diseases such as cancer, where USP25 and USP28 are often overexpressed. This compound is particularly useful in exploring potential therapeutic strategies aimed at disrupting tumor growth and enhancing the efficacy of cancer treatments. Additionally, AZ-1 is employed in biochemical assays to investigate the mechanisms of protein degradation and the ubiquitin-proteasome system, providing insights into cellular homeostasis and disease progression.