Used in research as a selective and potent inhibitor of deubiquitinating enzymes (DUBs), particularly targeting ubiquitin-specific protease 7 (USP7). It modulates the ubiquitin-proteasome system, making it a valuable tool compound in studies related to protein degradation, DNA damage response, and cancer cell signaling pathways. Due to its ability to stabilize p53 by disrupting USP7-MDM2 interactions, it is employed in oncology research to explore therapeutic strategies for tumors with wild-type p53. Its high specificity and cell permeability make it suitable for in vitro and cellular assays aimed at understanding epigenetic regulation and developing targeted cancer therapies.