Used in neuroscience research to study cholinergic systems, particularly in understanding the role of acetylcholine in synaptic transmission. It serves as a labeled compound in tracer studies, enabling precise tracking of neurotransmitter pathways. Applied in pharmacological experiments to investigate receptor binding and signal transduction mechanisms. Also utilized in metabolic studies to explore acetylcholine degradation and recycling processes. Its deuterated form enhances detection sensitivity in mass spectrometry and NMR analyses, making it valuable for advanced biochemical research.