Used as a stable isotope-labeled internal standard in gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analyses. Its fully deuterated structure (dodecane-D26, with 26 deuterium atoms) makes it ideal for tracing metabolic pathways and studying hydrocarbon behavior in complex mixtures. Commonly applied in environmental testing to quantify aliphatic hydrocarbons without interference from natural abundance signals. Also utilized in kinetic studies and as a non-reactive tracer in petroleum research due to its chemical similarity to dodecane while being distinguishable by mass detection.