N-Butyl Acetate-D12 is primarily used as a deuterated solvent in analytical chemistry and biochemistry, particularly in NMR (Nuclear Magnetic Resonance) and MS (Mass Spectrometry) techniques. Its deuterium isotope labeling (D12) enables precise molecular tracking, quantification, and structural analysis. It is also employed in pharmaceutical research and development to study chemical mechanisms and metabolic changes in the body without interfering with experimental results.