Used in molecular biology for dephosphorylation of DNA and RNA to prevent self-ligation during cloning. Commonly applied in restriction enzyme cloning and vector preparation. Also used in diagnostics as a label in immunoassays, especially in ELISA, due to its ability to produce detectable signals with chromogenic substrates. Plays a role in bone and liver function tests in clinical chemistry, where its activity levels help assess diseases like rickets, osteomalacia, and cholestatic liver conditions. Employed in research to study cell differentiation, particularly in stem cell and tissue mineralization studies.