Used in biomedical research to quantify soluble RANKL (receptor activator of nuclear factor kappa-B ligand) in biological samples such as serum, plasma, and cell culture supernatants. This ELISA kit helps investigate bone metabolism disorders including osteoporosis, rheumatoid arthritis, and bone metastasis by measuring sRANKL levels, which play a key role in osteoclast activation and bone resorption. It is also applied in immunology research since RANKL is involved in immune cell regulation and lymph node development. The assay supports drug development and evaluation of therapeutic agents targeting the RANKL/RANK/OPG pathway.